Figure 5
From: Regulation of cerebrospinal fluid production by caffeine consumption
The effect inversion of caffeine was associated with the induction of the A 1 adenosine receptor after long-term caffeine treatment. A. Representative photographs of adenosine A1 and A2A receptor immunohistochemistry in the choroid epithelial cells of the control and caffeine-treated rats (n = 4). Scale bar, 100 μm. B. Representative Western blots of the adenosine A1 and A2 receptors. C. Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. D. Representative MR images (n = 27) and photographs of Na+, K+-ATPase and AQP1 immunohistochemistry of the choroid epithelial cells of the control, CPA-treated and CGS21680-treated rats (n = 4). Scale bar, 100 μm. An A1 agonist, CPA, and an A2A agonist, CGS21680, were associated with ventriculomegaly. CPA but not CGS21680 was correlated with an increased expression of Na+, K+-ATPase but not AQP1. E. Representative Western blots of Na+, K+-ATPase and AQP1. F. Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. Values are expressed as a percentage of the control (n = 6). The results were analyzed by one-way ANOVA followed by Tukey's multiple comparison. *, P < 0.001; **, P < 0.05 versus control rats