Figure 2

Modulation of synaptic transmission over 10 hours after induction of AP bursting. (A) Stimulation protocol used to induce action potential bursting (see Methods). Bicuculline (bic, 50 μM) or vehicle was applied for 15 minutes. mEPSCs were recorded after washout at various time points over the next 10 hours. (B) Mean values of mEPSC IEI (left), mEPSC amplitude (centre) and whole-cell AMPA response (right) recorded from cells treated for 15 minutes with either vehicle or bicuculline and exhibiting either AP bursting or random/silent activity patterns (see legend). Recordings span a 10 hour time period after treatment. Cell numbers in each group are shown in the left histogram. Asterisks indicate significant differences compared with the bicuculline treated, action potential bursting group. (C) The IEI data shown in B is separated into 3 groups to illustrate the time course of its modulation. Numbers within columns represent n of cells per group. * p < 0.05, ** p < 0.01, *** p < 0.001.