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Figure 1 | BMC Neuroscience

Figure 1

From: Effects of estrogens and bladder inflammation on mitogen-activated protein kinases in lumbosacral dorsal root ganglia from adult female rats

Figure 1

Phosphorylation of p38 MAP kinase in cultured adult rat DRG neurons following treatment with ER agonists and antagonists (10 min). A, 17β-Estradiol (E2), PPT and DPN (each 10 nM) all stimulated p38 phosphorylation (separate cultures from 3 rats). A representative Western blot is shown below. B, E2 (10 nM) stimulated p38 phosphorylation that was prevented by tamoxifen (tx; 1 μM) and mimicked by ICI182,780 (ICI; 3 μM). The response to E2 with ICI182,780 was greater than with E2 alone. Tamoxifen alone had no effect. Data comprises separate cultures from 3 rats, expressing phosphorylated p38 relative to total p38 protein. A representative Western blot is shown below. * P < 0.05, ** P < 0.01, *** P < 0.001, relative to control; # P < 0.05 relative to E2.

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