Figure 1From: Effects of estrogens and bladder inflammation on mitogen-activated protein kinases in lumbosacral dorsal root ganglia from adult female ratsPhosphorylation of p38 MAP kinase in cultured adult rat DRG neurons following treatment with ER agonists and antagonists (10 min). A, 17β-Estradiol (E2), PPT and DPN (each 10 nM) all stimulated p38 phosphorylation (separate cultures from 3 rats). A representative Western blot is shown below. B, E2 (10 nM) stimulated p38 phosphorylation that was prevented by tamoxifen (tx; 1 μM) and mimicked by ICI182,780 (ICI; 3 μM). The response to E2 with ICI182,780 was greater than with E2 alone. Tamoxifen alone had no effect. Data comprises separate cultures from 3 rats, expressing phosphorylated p38 relative to total p38 protein. A representative Western blot is shown below. * P < 0.05, ** P < 0.01, *** P < 0.001, relative to control; # P < 0.05 relative to E2.Back to article page