Table 1 Nucleotide sequences for primers used in RT-PCR studies.
Target (Accesion no.) | Sequence (5'-3') | Amplicon size (bp) |
|---|---|---|
Gustducin (Gnat3) | AGTACTTCGCAACCACCTCCAT (F) | 249 |
   [Genebank: NM_001081143] | GTCACTGCATCAAACACAA (R) |  |
T1R2 (Tas1r2) | TAGGAAAAGACAGGGGGAGTGG (F) | 208 |
   [Genebank: NM_031873] | GGGGGTGTAGAGAAGCGAGAAT (R) |  |
TRPM5 | CTCCCAGCAGCCCCAAGAAATG (F) | 312 |
   [Genebank: NM_020277] | TGGGTCAGGGGTCAGAAAGAAA (R) |  |
PKD2L1 | GGTGAGATTCCAACAGAGG (F) | 202 |
   [Genebank: NM_181422] | CACCACATATTAGTCCAAAAGA (R) |  |
β-actin | CGTTGACATCCGTAAAGACC (F) | 244 |
   [Genebank: NM_007393] | AGGGGCCGGACTCATCGTA (R) |  |
GAPDH | AGGGCATCTTGGGCTACACTG (F) | 310 |
   [Genebank: NM_008084] | TATTATGGGGGTCTGGGATGGA (R) |  |
SCN1A | CTTTGCTGAGCTGGGTTTGT (F) | 227 |
   [Genebank: NM_018733] | GCAGGAGGAAGCGGGGATTTA (R) |  |
| Â | GTGACTGTGTAAAGGGGAGATA (F) | 331 |
| Â | CACACCGGGAAAAGAGTT (R) | Â |
SCN2A | ATGCGGGAAAATGCTATGAC (F) | 325 |
   [Genebank: NM_001099298] | TAACTTTCCACTACTCTACC (R) |  |
| Â | CAAGAGATTTCAAGGGGAGCAG (F) | 298 |
| Â | TTGGCAAGATCATTTCACTAAC (R) | Â |
SCN3A | AAAGCGGGTCCTGGGTGAG (F) | 336 |
   [Genebank: NM_018732] | GGAAGGAGGAGAGGTGGTAGAG (R) |  |
| Â | ACGCCACCCAGTTCATAGAGTT (F) | 290 |
| Â | CTTGTTTGCGCTTCAGAGTGGT (R) | Â |
SCN4A | GTGGGGCTCTACAGGGCTTGAC (F) | 271 |
   [Genebank: NM_133199] | CATGGGGGTGAGAGGAGTAG (R) |  |
| Â | TTGGGGAACGGGGCTTTGA (F) | 223 |
| Â | GCTGGGGGACCTGGGCTCTTA (R) | Â |
SCN5A | TGTCCCTCCCTGGCACTCACTT (F) | 278 |
   [Genebank: NM_021544] | TGCCCCTCCCTCCTTCCGTCTA (R) |  |
SCN7A | CCATGGCTGCGGGAGAC (F) | 239 |
   [Genebank: NM_009135] | CATTTTGCCTTAAGCGGTAGC (R) |  |
| Â | CCTTCTGTTGGGATTTCTTATT (F) | 325 |
| Â | ATGTCGAGGCAGTGGATTC (R) | Â |
SCN8A | GCAGATGGAGGAGCGGTTCGTG (F) | 279 |
   [Genebank: NM_001077499] | CCGCTGCTGCTTCTCCTTGTCG (R) |  |
| Â | TGGGAGACAGTGGGGAGTTGG (F) | 295 |
| Â | GTCGGGCTTTGTCACGCTGTCG (R) | Â |
SCN9A | AGCCCATACTAGCAGATTCCTC (F) | 291 |
   [Genebank: NM_018852] | TATATTCATGGCTACTTACTCA (R) |  |
SCN10A | AGAGATGGCACCAACCTACAG (F) | 302 |
   [Genebank: NM_009134] | ACCCCTATGCGACAGTGC (R) |  |
| Â | CGTTGGTCCCCGGAGATAAGAT (F) | 345 |
| Â | GGGAGCCCACCGTTGTCATTTG (R) | Â |
SCN11A | GCGAACAAGCGGCGGACTC (F) | 287 |
   [Genebank: NM_011887] | TACTTATGGCAGGGTTTTGACT (R) |  |
| Â | GGAGGAGGAATGTGCCGCTGTC (F) | 237 |
| Â | GTGAGTCCGCCGCTTGTTCGC (R) | Â |