Figure 4

Increased number of early newborn NeuroD- and DCX-expressing neurons in Per2^Brdm1mutant mice. DG of P45 WT (A;D;G) and Per2^Brdm1(B;E;H) mice were immunostained for NeuroD (A-B;G-H) and DCX (D-E). Total NeuroD⁺ (C) and DCX⁺ (F) cells per section were quantified (mean ± SD, n = 4, Student's t-test, *p < 0,05). (G-I) Assessment of the "short-term" neurogenesis. WT and Per2^Brdm1adult mice received a single injection of BrdU (i.p. injection at 100 mg/kg of body weight) and were sacrificed 4 days later. (G-H) DG sections were immunostained with anti-BrdU (green) and anti-NeuroD (red) antibodies. All images represent «Z-stack» overlays on 40 μm-thick coronal sections of 5 adjacent confocal planes (step size = 1 μm). (I) Histograms representing the assessment of the number of immature newborn BrdU⁺/NeuroD⁺ neurons per DG (mean ± SD, n = 4, Student's t-test, *p < 0,05). Scale bar in A = 50 μm for A-H.