Figure 5
From: Period 2 regulates neural stem/progenitor cell proliferation in the adult hippocampus
mPer2 mutation increased in vitro neural stem/progenitor cell proliferation and differentiation. (A-C) Representative neurosphere morphologies and size categories for DG of adult P45 WT and Per2Brm1mice. (C) Spheres were categorized into 3 size groups: (1) 50–100 μm, (2) 100–150 μm and (3)>150 μm in diameter. Data were obtained from three independent experiments and are expressed as mean ± SEM (***p < 0,001 and *p < 0,05 were analyzed by student's t-test). (D-F) Differentiating adult NSC cultures for 5 days in vitro on poly-L-ornithin and laminin immunostained for β-III tubulin (Tuj1, red) and DAPI (blue). Increased percentage of neurons was obtained for Per2Brdm1differentiated neurospheres as compared to WT (D). Results are expressed as mean ± SEM (n = 3 independent experiments, *p < 0,05 analyzed by student's t-test). Scale bar in A = 100 μm for A-B and 50 μm for E-F.