Skip to main content
Figure 1 | BMC Neuroscience

Figure 1

From: Bilobalide modulates serotonin-controlled behaviors in the nematode Caenorhabditis elegans

Figure 1

Serotonin stimulated egg-laying and survival assay. (A) Wild type young larvae (L1) were fed with bilobalide (BB) ranging from 3 nM to 3 μM for 4 days. The number of eggs was scored 60 minutes after 5-HT (5 mg/ml) was added in 96-well plates (*p < 0.05, **p < 0.001). (B) Egg-laying response to BB in wild type and 5-HT mutants adult day 1 worms. (C) Wild type adult day 1 worms (8 from each group) were fed with Bilobalide (BB) (0.3 μM) from L1 stage. Animals were then pre-incubated with dopamine for 2 h before being placed in a 5-HT solution. The bars represent the mean of the number of eggs from three individual assays; *p < 0.05. (D) Life span assay in N2 C. elegans fed either Bilobalide (BB) 0.3 μM (closed circle, bilobalide), WAY 100 635 (open squares, WAY 100 635) 0.3 μM or the vehicle control (open circle, Ctrl). Worms were transferred for 4 consecutive days during their egg-laying phase, and then every other day after the egg-laying phase was completed. Data are expressed as the percentage (%) of survival in an age-synchronized population. Three independent experiments were performed with number of worms totaling 200.

Back to article page