Figure 8

NET localizes to lipid rafts at the bouton surface but not to raft domains labeled within axonal membranes. SCGs were double-labeled with NET-05 and with fluorescent cholera toxin B (CTx-B). SCG's were either labeled with CTx-B prior to (A-D), or after detergent permeabilization (E-H), followed by NET-05 labeling. Small arrows indicate colocalization whereas large arrows point to regions of segregation. Correlation of NET and CTx-B pixel intensities using nonpermeabilized conditions (M, upper left), ICA analysis (lower panels) and spatial display of PDM values (M, upper right). N. Correlation of NET and CTx-B pixel intensities using permeabilizing conditions (upper left), ICA analysis (lower panels) and spatial display of PDM values (N, upper right). Scale bar = 5 μM. PDM color transformation is shown above standard red/green visualizations of same field.