Figure 3
From: Calyx and dimorphic neurons of mouse Scarpa's ganglion express histamine H3 receptors
Immunofluorescence of calretinin and histamine H3 receptor in mouse Scarpa's ganglion. A: confocal image of a Scarpa's ganglion slice immunolabeled for H3 receptors. B: Immunolabeling for calretinin, same field as in A. C: nuclei counterstained with DAPI, same field as in A and B. D: overlapped images; yellow indicates the colocalization of calretinin and H3 receptors (three examples shown by arrows). Calibration bar: 10 μm. Cryostat slices thickness: 5 μm. Green: H3R Alexa Fluor 488; Red: Calretinin Alexa Fluor 546; Blue: DAPI. a, b, c and d are larger magnifications from a different slice, same antibodies and description as above. Calibration bar: 20 μm.