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Table 2 The effect of adaptor protein signaling at Y490 TrkB for Kv1.3 current properties.

From: Brain-derived neurotrophic factor modulation of Kv1.3 channel is disregulated by adaptor proteins Grb10 and nShc

Transfection Condition

Peak Current

(pA)

Ï„Inact

(msec)

Ï„Deact

(msec)

Kv1.3 + TrkB

   

   Pre

356 ± 71 (5)

697 ± 130

32.2 ± 2.1

   Post

*298 ± 64

780 ± 59

24.2 ± 2.9

Kv1.3 + TrkBShc-

   

   Pre

372 ± 144 (3)

693 ± 45

31.8 ± 3.3

   Post

386 ± 140

843 ± 114

26.5 ± 3.7

Kv1.3 + TrkBShc- + nShc

   

   Pre

533 ± 109 (10)

1070 ± 163

31.2 ± 4.3

   Post

473 ± 115

1040 ± 196

35.3 ± 3.8

Kv1.3 + TrkBShc- + Grb10

   

   Pre

533 ± 122 (4)

894 ± 173

29.0 ± 1.6

   Post

*379 ± 84

660 ± 229

32.6 ± 7.8

  1. HEK 293 cels were transfected with cDNAs encoding Kv1.3 +TrkB kinase or TrkBShc- (Y490 mutation) ± adaptor protein. Several voltage-stimulating paradigms were completed (see "Methods") to collect the tabled biophysical properties by cell-attached patch-clamp recording. BDNF stimulation, notations, and statistical analysis as in Table 1.