c-MycERTAM transgene silencing in a genetically modified human neural stem cell line implanted into MCAo rodent brain

Stevanato, Lara; Corteling, Randolph L; Stroemer, Paul; Hope, Andrew; Heward, Julie; Miljan, Erik A; Sinden, John D

doi:10.1186/1471-2202-10-86

Table 1 c-MycER^TAM transcript copy number and number of CTX0E03 cells calculated in the grafted rat brain sections at 1- and 4-weeks post-implantation

From: c-MycER^TAM transgene silencing in a genetically modified human neural stem cell line implanted into MCAo rodent brain

Week 1					Week 4
Rat# region	Total c- mycER^TAM copy #	Total cell # Alu assay	Total c -mycER^TAM copy #/Total cell # Alu assay	c -mycER ^TAM copy # per cell per animal	Rat# region	Total c -mycER^TAM copy #	Total cell # Alu assay	Total c -mycER^TAM copy #/Total cell # Alu assay	c -mycER ^TAM copy # per cell per animal
201 F1	N.D.¹	N.D.²	N/A		203 F1	N.D.	N.D.	N/A
201 F2	N.D.	1066.9	0.0		203 F2	N.D.	N.D.	N/A
201 FN1	18671.8	18821.5	1.0		203 FN1	N.D.	1776.9	0.0
201 FN2	N.D.	11695.4	0.0	0.8	203 FN2	N.D.	15046.2	0.0
301 F1	N.D.	530.8	0.0		203 C	521495.6	68123.1	7.7	6.1
301 F2	N.D.	356.8	0.0		204 F1	902695.4	45261.5	20.0
301 FN1	N.D.	16269.2	0.0		204 F2	N.D.	5563.1	0.0
301 FN2	N.D.	10993.9	0.0	0.0	204 FN1	N.D.	4850.8	0.0
302 F1	N.D.	1075.4	0.0		204 FN2	418067.7	45661.5	9.2	13.0
302 F2	N.D.	837.7	0.0		403 F1	N.D.	N.D.	N/A
302 FN1	909865.9	42992.3	21.1		403 F2	264100.0	5501.5	48.0
302 FN2	N.D.	18664.6	0.0	14.3	403 FN1	388332.3	85000.0	4.6
501 F1	N.D.	N.D.	N/A		403 FN2	224224.0	17169.2	13.1
501 F2	550.1	17846.2	0.0		403 C	1324893.5	47584.6	27.8	14.2
501 C1	2858994. 9	123000.0	23.2		404 F1	N.D.	2738.5	0.0
501 C2	N.D.	2701.5	0.0	19.9	404 F2	31223.6	11076.9	2.8
601 F1	N.D.	N.D.	N/A		404 FN1	N.D.	146750.0	0.0
601 F2	N.D.	964.2	0.0		404 FN2	218605.5	15061.5	14.5
601 FN1	10151.6	70061.5	0.9		404 F ANT	N.D.	3556.9	0.0	0.4
601 FN2	109810.0	18692.3	5.9	1.3	502 F1	N.D.	241.4	0.0
602 F1	380824.6	41123.1	9.3		502 F2	N.D.	1046.2	0.0
602 F2	N.D.	24076.9	0.0		502 FN1	150198.4	12776.9	11.7
602 C	40085.1	11253.9	3.6		502 FN2	224974.8	19261.5	11.7	11.3
602 FL	N.D.	6541.5	0.0	5.1	503 F1	N.D.	N.D.	N/A
					503 F2	N.D.	N.D.	N/A
					503 FN1	N.D.	1230.8	0.0
					503 FN2	N.D.	13292.3	0.0
					503 C	N.D.	19692.3	0.0
			Mean	6.9				Mean	7.7
			SEM	3.4				SEM	2.5

¹N.D. Not Detected, signal was below the level of 10 c-mycER^TAM copies per reaction
²N.D. Not Detected, signal was below the level of one CTX0E03 cell per reaction

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ISSN: 1471-2202

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