Figure 7From: Non-invasive evaluation of nigrostriatal neuropathology in a proteasome inhibitor rodent model of Parkinson's disease(A-D) α-synuclein immunohistochemistry reveals the presence of soluble aggregates of α-synuclein in the substantia nigra of lactacystin-lesioned animals compared to saline controls (panels A, B: ×4 magnification, scale bars = 200 μm; panels C, D: ×40 magnification, scale bars = 20 μm). Note the diffuse pattern of staining in control animals compared to the punctate labelling observed in lactacystin-lesioned animals, indicative of aggregation. (E-F) Pre-treatment with proteinase K (10 μg) reveals the presence of insoluble perinuclear α-synuclein aggregates in the SNc of lactacystin-injected animals, but not saline controls (×60 magnification, scale bars = 10 μm).Back to article page