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Figure 2 | BMC Neuroscience

Figure 2

From: Cellular toxicity following application of adeno-associated viral vector-mediated RNA interference in the nervous system

Figure 2

Efficient lentivirus mediated knockdown in F11 cells. F11 cells were infected with a lentiviral vector expressing GFP and a control shRNA and a two shRNAs directed against Npn-1 or Npn-2. Two days after infection Npn-1 mRNA expression was reduced to 31.4 ± 1.8% and 17.5 ± 3.4% (mean ± SEM). Npn-2 expression was knocked down to 7.8 ± 1.1% and 13.3 ± 1.0% (mean ± SEM) (A). Western blot (D) and quantification thereof (B) for Npn-1 showed a similar knockdown efficiency 39.2 ± 9.7% and 5.7 ± 2.6% (C) (mean ± SEM) (* <0.05, ** p < 0.005)

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