Figure 2From: Acute NMDA toxicity in cultured rat cerebellar granule neurons is accompanied by autophagy induction and late onset autophagic cell death phenotypeQuantification of punctate stains in LC3-positive neurons. Punctate stains were quantified in different microscopic fields (n = 4) and compared to neurons exhibiting densely stained LC3 immunopositive autophagosomes. The numbers were plotted and statistical analysis was performed using Student's t-test. *p < 0.05 denotes significant differences between different treatment groups to serum free medium treated control neurons (Ctrl). # p < 0.05 denotes statistical significance in treatment groups when compared to NMDA-treated neurons alone.Back to article page