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Figure 6 | BMC Neuroscience

Figure 6

From: Acute NMDA toxicity in cultured rat cerebellar granule neurons is accompanied by autophagy induction and late onset autophagic cell death phenotype

Figure 6

NMDA-induced autophagosome formation is inhibited by 3-MA. Representative fluorescence micrographs show an increase in Monodansylcadaverine (MDC; 0.05 mM) labeling of autophagosomes in neurons increase following acute exposure to NMDA (200 μM) compared to control conditions and co-treatment of NMDA+3-MA. Yellow arrows and yellow arrow heads indicate the presence of regularly sized autophagosomes in the cell bodies and neurites respectively in 12 and 16 hours after NMDA exposure. The red arrows in the image at 16 hours are indicative of the accumulation of the unusually large MDC-positive autophagosomes in the cell bodies of neurons following prolonged NMDA exposure. Images are taken at 400× magnification. Scale bar represents 20 μm.

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