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Figure 9 | BMC Neuroscience

Figure 9

From: Dynamics of peptidergic secretory granule transport are regulated by neuronal stimulation

Figure 9

Fusion events are detected by increases in PHM-pHluorin fluorescence. Neurons were co-transfected with vectors encoding PHM-pHluorin and DsRed. All images in this figure are from a single microscope field of one neuron; four other neurons yielded similar results. After 2 days in culture, growth cones of neurons expressing DsRed were identified, and image frames were acquired under basal conditions (A, top left). A horizontally oriented line was placed through the center of the axon and growth cone. Scans were acquired continuously along the indicated line during PMA stimulation; two examples are shown (C, left and middle). Distance in the x dimension is represented on the x-axis, with the cell soma out of the picture to the left and the growth cone to the right. Time is represented on the y-axis; the total time for each image displayed is 6 sec. At the conclusion of the experiment, the total LDCV population was visualized by adding 5 mM NH4Cl to the bath solution; a frame (B, right) and line scan (C, right) are shown.

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