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Figure 1 | BMC Neuroscience

Figure 1

From: Interaction of the mu-opioid receptor with GPR177 (Wntless) inhibits Wnt secretion: potential implications for opioid dependence

Figure 1

Directed Y2H screen. The carboxyl-terminus of GPR177 (residues 451-541) was used as bait in a directed Y2H assay for interaction with each of the intracellular loops (IL) and carboxyl-terminus (C-tail) of the mu-opioid receptor (MOR) and the second intracellular loop of the D2 dopamine receptor (D2IC2). Full-length CAPS (calcium activated protein for secretion), a known interactor of the D2IC2 was used as a positive control, and empty bait vector (pACT2) was used as a negative control. A positive interaction is indicated by the production of a blue colony in the β-gal assay.

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BMC Neuroscience

ISSN: 1471-2202

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