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Figure 1 | BMC Neuroscience

Figure 1

From: Multimodal investigations of trans-endothelial cell trafficking under condition of disrupted blood-brain barrier integrity

Figure 1

Schematic representation of the surgical procedure and quality control of Gd++-TALL-104 cells. A) Experimental steps common to all the experiments. BBB disruption (BBBD) was performed after baseline MRI. Continuous electroencephalography (EEG) and trans-cranial Doppler (TCD) recordings were performed. MRI scans were performed before and 48 hours after the procedures. B) Catheter was placed in the femoral artery and angiographically guided to the ICA at the base of the brain. C1) Localization of the tip of the catheter and angiographic exploration. Note the extent of contrast diffusion to the hemisphere correspondent to the ICA cannulated and a modest spread to the contralateral side. Note also the venous return after contrast injection. C2) Successful BBBD lead to hemispheric leakage of Evan's Blue in the brain parenchyma. Note the negligible amount of blue signal in the contralateral side. D) Cell vitality was demonstrated by lack of Trypan Blue penetration. E) MRI scans (T1) confirmed that TALL-104 cells retain Gd++.

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