Figure 3

FRAP indicating the mobility of vesicles in response to application of the Myosin inhibitor, ML-9. a, Acquired images for the recovery of fluorescently labelled vesicles using elav^C155Gal4;UAS-sytGFP/Y after application of ML-9. Images are shown immediately before bleaching (8 sec), immediately after photobleaching (10 sec) and at 16, 48 and 120 sec. Bleached areas are indicated by white arrows. A reduction in recovery is observed with increasing doses of ML-9. b, A significant dose-dependent response was observed for inhibition with ML-9 between the doses of 100 μM ML-9 and 10 μM ML-9 as compared to 0 μM ML-9. 10 μM ML-9 did not significantly reduced vesicle mobility. Error is represented as the 95% confident interval of the curve. FRAP recoveries were fit with double exponential curves and nonlinear regression was used to test for statistical differences. Sample sizes and significance were as follows: 0 μM ML-9 (n = 20), 10 uM ML-9 (n = 22, p > 0.05), 50 μM ML-9 (n = 20, p < 0.05), 100 μM ML-9 (n = 20, p < 0.05).