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Figure 4 | BMC Neuroscience

Figure 4

From: Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophilaneuromuscular junction

Figure 4

FRAP recovery curves for vesicle mobility when NMMII expression is altered. a, Acquired images for the recovery of the heterozygous loss-of-function, elavC155Gal4;UAS-sytGFP/+; zip1/+ (Het), the control, elavC155Gal4;UAS-sytGFP/Y (ct), the RNAi knockdown, elavC155Gal4;UAS-sytGFP/+;UASzipRNAi/+ (K/D) and the overexpression, elavC155Gal4;UAS-sytGFP/+; zipGS50077/+ (O/E) of NMMII immediately before bleaching (8 sec), immediately after photobleaching (10 sec) and at 12, 16, 48 and 120 sec post-bleaching. Bleached areas are indicated with white arrows. At 16 sec the bleached region for Het is no longer visible; however, it is still clearly visible in control (ct). At 120 sec, the bleached region is no longer visible in the control (ct), but is still visible for both K/D and O/E. b, Vesicle mobility is affected by the expression level of NMMII. FRAP curves reveal that the heterozygous NMMII loss-of-function (Het) significantly enhanced vesicle mobility as compared to the control (ct) while both knockdown (K/D) and overexpression (O/E) of NMMII significantly reduced vesicle mobility. FRAP recoveries were fit with double exponential curves. Nonlinear regression was used to test for statistical differences; elavC155Gal4;UAS-sytGFP/Y (n = 26), elavC155Gal4;UAS-sytGFP/+; zip1/+ (n = 24, p < 0.05), elavC155Gal4;UAS-sytGFP/+;UASzipRNAi/+ (n = 22, p < 0.05), elavC155Gal4;UAS-sytGFP/+; zipGS50077/+ (n = 20, p < 0.05).

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