Figure 4From: Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophilaneuromuscular junctionFRAP recovery curves for vesicle mobility when NMMII expression is altered. a, Acquired images for the recovery of the heterozygous loss-of-function, elavC155Gal4;UAS-sytGFP/+; zip1/+ (Het), the control, elavC155Gal4;UAS-sytGFP/Y (ct), the RNAi knockdown, elavC155Gal4;UAS-sytGFP/+;UASzipRNAi/+ (K/D) and the overexpression, elavC155Gal4;UAS-sytGFP/+; zipGS50077/+ (O/E) of NMMII immediately before bleaching (8 sec), immediately after photobleaching (10 sec) and at 12, 16, 48 and 120 sec post-bleaching. Bleached areas are indicated with white arrows. At 16 sec the bleached region for Het is no longer visible; however, it is still clearly visible in control (ct). At 120 sec, the bleached region is no longer visible in the control (ct), but is still visible for both K/D and O/E. b, Vesicle mobility is affected by the expression level of NMMII. FRAP curves reveal that the heterozygous NMMII loss-of-function (Het) significantly enhanced vesicle mobility as compared to the control (ct) while both knockdown (K/D) and overexpression (O/E) of NMMII significantly reduced vesicle mobility. FRAP recoveries were fit with double exponential curves. Nonlinear regression was used to test for statistical differences; elavC155Gal4;UAS-sytGFP/Y (n = 26), elavC155Gal4;UAS-sytGFP/+; zip1/+ (n = 24, p < 0.05), elavC155Gal4;UAS-sytGFP/+;UASzipRNAi/+ (n = 22, p < 0.05), elavC155Gal4;UAS-sytGFP/+; zipGS50077/+ (n = 20, p < 0.05).Back to article page