Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophilaneuromuscular junction

BMC Neuroscience

Table 1 Rate parameters from FRAP curves fit with the double exponential curves

Treatment	Rate Parameters¹
	A	K1	B	K2
Vesicle Mobility
0 μM ML-9	0.38 ± 0.031	0.0040 ± 0.0010	0.72 ± 0.031	0.11 ± 0.40
10 μM ML-9	0.37 ± 0.0035	0.0035 ± 0.0010	0.40 ± 0.083	0.080 ± 0.023
50 μM ML-9	0.39 ± 0.030	0.0025 ± 0.0010	0.42 ± 0.087	0.080 ± 0.022
100 μM ML-9	0.51 ± 0.020	0.0025 ± 0.0005	0.29 ± 0.16	0.11 ± 0.050
Control (ct)	0.40 ± 0.033	0.0047 ± 0.0012	0.58 ± 0.22	0.10 ± 0.039
Heterozygous Loss-of-Function (Het)	0.37 ± 0.043	0.0069 ± 0.0015	0.66 ± 0.33	0.12 ± 0.049
RNAi Knockdown (K/D)	0.46 ± 0.035	0.0031 ± 0.0008	0.28 ± 0.12	0.082 ± 0.045
Overexpression (O/E)	0.49 ± 0.040	0.0023 ± 0.0010	0.35 ± 0.093	0.071 ± 0.030

¹ Equation: Frap(t) = 1-A(e^{-K1 off t})-B(e^{-K2 off t}) [38] where A, B = constants that represent the apparent bleached fraction, K1, K2 = rates, t = time

ISSN: 1471-2202