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Figure 9 | BMC Neuroscience

Figure 9

From: Acute stress alters transcript expression pattern and reduces processing of proBDNF to mature BDNF in Dicentrarchus labrax

Figure 9

Stress increases significantly the percentage of proBDNF while decreasing matBDNF in the brain of stressed seabass. A) The quantification of total BDNF in the brain of seabass using an ELISA assay, showed no difference between control and stressed group (A.I.U. = arbitrary intensity units). B) A representative Western blot of proBDNF and mature BDNF expression in the seabass brain. Human proBDNF and mature BDNF is shown in the first lane from left. Alpha-tubulin used for normalization is shown at the bottom. C) ProBDNF on total BDNF is significantly increased (** = p < 0.01) in stressed animals with respect to control animals and mature BDNF percentage is increased (** = p < 0.01; n = 15 animals, each in duplicate. Error bars are SE). D) The scatterplot shows that in the brain of all stressed animals proBDNF are above the mean of controls+1SD. E) Similarly, in all stressed animals mature BDNF values are below the mean of controls -1SD. Only 2 control animals have a proBDNF percentage above the controls' mean+1SD for proBDNF or below the mean-1SD. The F values, obtained by One way Anova analysis, for proBDNF and mature BDNF were 19.028 and 6.225, respectively.

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