| NAME | SEQUENCE 5'-3' | Tm°C | Notes |
---|---|---|---|---|
Genome Walking | 1beta_SP1 | CCACTGAGTCCAACCCTTCCAGCAATGC | 72.2 | 3 |
 | 1beta_SP2 | ACCATTTTCCCCTACGCTGTCCTGGAGATAG | 70.2 | 3 |
 | 1beta_SP3 | ACTTCTGCTGTGCTCAGTAGATCGCCCACC | 71.9 | 3 |
 | 1beta_SP4 | GGCAAATATCAACAAGCCCGGGTTGTCAG | 72.0 | 3 |
 | 1beta_SP5 | GGCAATCCAAGTTTGTGGGGGTACTAGTTC | 68.3 | 3 |
 | 1beta_SP6_A | GAGTGTTAACTCCCTCTTTGGCGAGGGG | 69.7 | 3 |
 | 1beta_SP6_B | GGCCTATTACGCATACGCACAAACTGGTC | 69.0 | 3 |
 | AP1 | GTAATACGACTCACTATAGGGC | 59.0 | 4 |
 | AP2 | ACTATAGGGCACAGCGTGGT | 71.0 | 4 |
Genomic PCR | Dl_ex1beta_fw | CCAAGTGGTGGGCGATC | 57.6 | 2 |
 | Dl_ex1c_fw | CCATGCAATTTCCACCATC | 54.5 | 2 |
 | Dl_ex1a_fw | GTTAACTTTGGGAAATGCAAG | 54.0 | 2 |
 | Dl_P1_ant_BDNF | CCATAGTAACGAACAGGATG | 55.3 | 2 |
 | Dl_P2_ant_BDNF | GTCATCACTCTTCTAACCTGTTG | 58.9 | 2 |
 | Dl_ex1a_rev | CACTTGCATTTCCCAAAGTTAAC | 54.0 | 2 |
 | Dl_ex1c_rev | GATGGTGGAAATTGCATGG | 54.5 | 2 |
 | Int1c/2_FW | CCAGACAGTTTCTGTATTGTTGTTTTGGAGGGG | 70.2 | 3 |
 | Int1c/2_REV | TCCAGCCATGTGAGGATCAATTGTGAACGG | 73.6 | 3 |
 | Int1c/2_up | GTATTTTCTTAATTGCACACAGCGTGGGTGGG | 71.4 | 3 |
 | Int1c/2_low | CATTCTTAATTGGTATCTGGGGCCGTGGC | 71.0 | 3 |
 | Int1c/2_FWnew | CTCTAGGTGCGTTGTCATGCACAAAGGC | 69.8 | 3 |
 | Int1c/2_REVnew | AGGGGTAATATTGCAGTAGCAGGGGGTGG | 70.0 | 3 |
 | Int1a/1c_FW | ATGCTCCCAATATGGGACCTTAAGACGCTGC | 72.2 | 3 |
 | Int1a/1c_FWnew | GTAATCGTTGCGTTGTGCTTAATCATGCTCC | 66.8 | 3 |
 | Int1a/1c_FW1 | GGTCTGCTGCATTCATGTTTTGTCTTGATG | 68.7 | 3 |
 | Int1a/1c_FW2 | GCCCTACTCTTTACCCCCCCCACCC | 70.2 | 3 |
 | 1crev_SP1 | TCTCCCGACAAGCTTCAGGATATCTCTTCAGC | 70.8 | 3 |
 | Int1a/1c_REV_1 | TTTTGCGTAACIGCGCGTCTCCACCAIGTC | 74.5 | 3 |
 | Int1a/1c_REV_2 | CAAACTCCTGGATATGAGCTTAAAGGAGGC | 66.4 | 3 |
 | Int1a/1c_REV_3 | CGTTTGGCATGTAGCAGTATGGGAGTGG | 69.3 | 3 |
 | Int1a/1c_REV_4 | CCTTTCAAGGCTTCTCTTGCCAAATGC | 68.2 | 3 |
 | Int1a/1c_REV_5 | CATCCTGCCAGCATGTGCAACTGC | 69.6 | 3 |
 | Int1a/1c_REV_6 | CCTGACTCACTTTTAGCCTATCTGACATGCAGG | 69.3 | 3 |
 | Int1a/1c_REV_7 | CACACACACACACACACACACACACTGTG | 68.3 | 3 |
 | Int1beta_FW | GACCAGTTTGTGCGTATGCGTAATAGGCC | 69.0 | 3 |
3'-RACE | SP1FW_3' | GGCTGCAGAGGAATAGACAAGCGGCAT | 70.4 | 3 |
 | SP2FW_3' | CCAATGCAGGACAACCCAGTCCTACGT | 69.3 | 3 |
 | BDNF_3'race | GACCATTAAGAGGGGCAGATAG | 60.3 | 3 |
 | AP | GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT | 71.1 | 4 |
 | UAP | CUACUACUACUAGGCCACGCGTCGACTAGTAC | 64.3 | 4 |
5'-RACE | RACE_BDNF_GSP1 | CTTGGTTGCTGATCATC | 50.4 | 3 |
 | RACE_BDNF_GSP2 | CTGTGAGTGAGGGCAGTTC | 58.8 | 3 |
 | RACE_BDNF_GSP3 | CGAACAGGATGGTCATCACTC | 59.8 | 3 |
 | AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | > 75.0 | 4 |
 | AUAP | GGCCACGCGTCGACTAGTAC | 66 | 4 |
RT-PCR | Dl_BDNF_up | ATGACCATCCTGTTCGTTAC | 55.3 | 2 |
 | Dl_BDNF_down | CTATCTGCCCCTCTTAATG | 54.5 | 2 |
 | 1beta_FW_nested | GCGAGGGTGTTACGTATATCTG | 58.7 | 3 |
 | 1beta_Rev_new | CCACTCACTCCAACAGATGC | 59.3 | 3 |
 | 1a_FW_new | GCTTATTCTGAGGGAGCCTG | 59.0 | 3 |
 | 1a_Rev_new | CCCAAAGTTAACGCAGTGTG | 59.2 | 3 |
 | 1bFW | CTCAGCTCTGCAGAGTTGGGGT | 61.8 | 3 |
 | 1c_FW_new | CGTTTCACCATGCGACAAC | 61.1 | 3 |
 | 1c_Rev_new | GCCCAGTCGTAAAACAGACC | 59.6 | 3 |
 | 1dFW | GTCCTGATGGAAACAGGAAATCAC | 63.1 | 3 |
 | 1dRev | CACAGATGACGTCTCTTCCAGGT | 62.9 | 3 |
 | Ex2_FW_new | CTTCAGTTGCATGAGAGCTGC | 61.3 | 3 |
 | Ex2_Rev_new | ACCCTCATGCACATATTAGCG | 60.0 | 3 |
 | Dl_BDNFreal_low | TTGCTTCAGTTGGCCATTGG | 57.3 | 3 |
 | Dl_Act_FW_RT | GGTATTGTCATGGACTCCGGTGAT | 61.9 | 1 |
 | Dl_Act_Right | TTAGAAGCATTTGCGGTGGA | 58.0 | 1 |
 | D.l._GAPDH_FW | GAGGGTGACAAGCTGGTCGT | 58.8 | 1 |
 | D.l._GAPDH_Rev | CAAAGATGGAGGAGTGAGTGTCAC | 58.8 | 1 |
Universal Primers | T7 | TAATACGACTCACTATAGGG | 53.2 | Â |
 | SP6 | CATTTAGGTGACACTATAG | 50.2 |  |