Figure 2

TRPC2/RTP1 co-localization in the rat nasal cavity. Sixteen micron coronal sections were processed for αRTP1 immunocytochemistry using an avidin-peroxidase chromagen method (A-F) or dual-colored immunocytochemistry with fluorescent secondary antisera (G-L). Low-power magnification of the (A) main olfactory epithelium (MOE) and (B) vomeronasal epithelium. osn = olfactory sensory neuron, res = respiratory epithelium, vsn = vomeronasal sensory neuron, gob = goblet cells, sp = soft palate. Higher-power magnification reveals αRTP1 labelling in the cilia layer of the MOE (inset C) and microvilli layer of the VNO (inset E) but absence of label in the respiratory cilia (inset D). Control VNO section with omission of primary antiserum (F). Higher-power magnification of the VNO (G-L), αRTP1 (G), αTRPC2 (H), merged image (I). Control sequential sections to that of G-I with omission of the primary antisera (J-L). Scale bar 25 μm (C-F) or 100 μm (A, B, G-L). Scale bar in J is the same for G-L. Italicized lettering in boxes is linked to enlarged image sub-panel. m = microvillar layer.