Figure 5

Subcellular localization of TRPC2 in HEK293 cells. (A-E) Representative two-photon confocal micrographs of cells grown, transfected, and immunolabeled as in Fig. 4. Note that TRPC2 immunoreactivity in (A) appears subcellular while that of Kv1.3 immunoreactivity (D) is localized to or compartmentalized near the membrane. The presence of RTP1 (B) or REEP1 (B-C) appears to shift some TRPC2 immunoreactivity towards the membrane while that in the presence of REEP1 (C) remains more cytosolic or intermediate. Immunoreactivity is abolished in a vector-only transfection in E (pcDNA₃). Horizontal scale bar = 10 μm. Vertical scale bar = 50 arbitrary units. (A-E) = Z-series stacked confocal image; (A'-E') = Representative single optical slice in the midline; (A''-E'') = Intensity profiles quantified as a line scan taken at the horizontal bar in A'-E'.