Figure 2From: A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neuronsConfirmation of Dcx expression in cerebellum extracts and embryonic stem cells during neuronal differentiation. ESR1 cells were subjected to detergent extraction as described in Materials and Methods at day of differentiation 0 (DD0), DD4, DD6, DD8 (on gelatin) and DD10 (on poly-ornithine/laminin). 20 μg of total protein were separated on 10% SDS-PAGE gels, immunoblotted and reacted with the corresponding antibodies; (upper panel) anti-Doublecortin and (lower panel) anti-α-Tubulin.Back to article page