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Table 3 Primers used for site-directed mutagenesis.

From: A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons

Mutated site

Primer (5'- 3')

Sequence

Ref

pdcx Lef*

Sense

ATCTCTCTATGCCGTGTTTGTCTGAGCTCCAAGC

[60]

 

Antisense

GCTTGGAGCTCAGACAAACACGGCATAGAGAGAT

 

pdcx Hnf6*

Sense

AGGCATAGAGAGCGTTTGTTTCTTTCTCTCAGCATCTCCACCCAACC

/

 

Antisense

GGTTGGGTGGAGATGCTGAGAGAAAGAAACAAACGCTCTCTATGCCT

 

pdcx Nf-y 1 *

Sense

AGGCATAGAGAGCGGTTTTTTCTTTCTCTCAGCTCTCCACCC

[61]

 

Antisense

GGGTGGAGAGCTGAGAGAAAGAAAAAACCGCTCTCTAGCCT

 

pdcx Nf-y 2 *

Sense

CGTTGTTTCTGCTGGTTTTGGGTGGAGATGC

[61]

 

Antisense

GCATCTCCACCCAAAACCAGCAGAAACAACG

 
  1. PCR fragments carrying the mutation are obtained by annealing the following primers in the pdcx 249bp-Luc plasmid.