Figure 3
From: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates
Cell proliferation and cell death assays. (A) and (C): Graphs of representative linear correlations in ATP assay performed in aNS-1 proliferating cells. 8 × 103 cells/well plated in 96-well microplates (A) and 4 × 103 cells/well plated in 384-well microplates (C); data are expressed as growth (%), referred to the value obtained at t = 0. The data are the mean ± SD from three independent experiments, each one performed in triplicate. (B) and (D): Data dispersion around mean value (full line) from ATP assays performed in 18 wells from a 96- (B) or a 384- (D) well microplate; upper and lower groups represent 48 h and 24 h data respectively; letters in x axes identify each individual well; dot lines represent 2SD from mean values. (E): Activation of Caspases 3/7 in LC1 cells exposed to the indicated doses of staurosporine for 6 hours. Data are normalized on cell viability, as assessed by CellTiter-Blue Cell Viability Assay (F): Activation of Caspases 3/7 in LC1 cells exposed to the indicated stress conditions for 48 hours. Data are normalized on cell viability. RLU: Relative Luciferase Unit.