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Figure 5 | BMC Neuroscience

Figure 5

From: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates

Figure 5

An YFP based assay for the measurement of GABAA receptor activity. (A) and (B): Extracellular Cl- overload produces a rapid decrease in cellular fluorescence that reaches the minimum in 7 sec. The fluorescence decrease is reversible, with complete recovery of resting fluorescence occurring within 2 minutes after removal of extracellular Cl-. (C): 21DIV aNS-1 YFP* cells have been exposed to GABA 20 mM or PBS only as a control. Fluorescence emission intensity has been recorded via AttoVision (BD Bioscience) every 5 seconds. Data elaboration through the integration of the area under the curve shows a decrease of the fluorescence signals specifically in the cells exposed to GABA.

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