Table 2 Relative proportion of aNS-1 cells expressing neural progenitor, neuronal and glial antigens during differentiation in 96-well microplates

DIV3

1

2

3

4

5

6

7

18

23

25

32

32

33

39

0 (n = 3074)

6.06 ± 3.34 (n = 608)

n.a.

n.a.

n.a.

10.00 ± 1.60

1.90 ± 0.52 (n = 1805)

52.22 ± 3.10 (n = 3134)

57.12 ± 14.03 (n = 661)

n.a.

n.a.

n.a.

51.47 ± 10.63

60.93 ± 0.63 (n = 2990)

64.13 ± 9.63 (n = 3074)

63.80 ± 8.12 (n = 608)

n.a.

n.a.

n.a.

45.60 ± 17.26

74.35 ± 4.71 (n = 1805)

0.17 ± 0.08 (n = 3134)

0.55 ± 0.61 (n = 661)

n.a.

n.a.

n.a.

5.30 ± 2.55

0.09 ± 0.15 (n = 2990)

DIV7

1

2

3

4

5

6

7

18

23

25

32

32

33

39

0.4 ± 0.07 (n = 4711)

1.36 ± 1.05 (n = 659)

3.14 ± 1.61

8.58 ± 3.72 (n = 366)

4.50 ± 2.02 (n = 367)

n.a.

1.64 ± 0.37 (n = 3791)

54.26 ± 1.59 (n = 3639)

59.91 ± 16.55 (n = 695)

40.27 ± 6.79

65.40 ± 14.70 (n = 470)

69.58 ± 10.74 (n = 453)

n.a.

66.86 ± 3.97 (n = 3666)

61.06 ± 7.56 (n = 4711)

61.31 ± 7.59 (n = 659)

39.02 ± 1.62

72.62 ± 9.71 (n = 366)

63.98 ± 12.95 (n = 367)

n.a.

74.89 ± 1.68 (n = 3791)

6.61 ± 2.58 (n = 4711)

2.13 ± 1.36 (n = 695)

5.33 ± 2.19

6.81 ± 8.91 (n = 470)

11.96 ± 4.46 (n = 453)

n.a.

0.43 ± 0.23 (n = 3666)

DIV14

1

2

3

4

5

6

7

18

23

25

32

32

33

39

0.40 ± 0.28 (n = 4475)

30.36 ± 3.61 (n = 715)

2.75 ± 2.15

7.39 ± 4.36 (n = 361)

4.57 ± 2.81 (n = 428)

3.49 ± 1.67

0.61 ± 0.37 (n = 2383)

40.94 ± 4.28 (n = 3416)

59.45 ± 9.59 (n = 636)

57.45 ± 7.19

66.05 ± 20.84 (n = 346)

77.40 ± 15.95 (n = 413)

43.52 ± 4.19

82.57 ± 3.86 (n = 2584)

74.25 ± 17.00 (n = 4475)

87.29 ± 6.77 (n = 715)

61.35 ± 7.16

61.67 ± 12.06 (n = 361)

62.67 ± 13.52 (n = 428)

52.72 ± 2.42

75.35 ± 5.32 (n = 2383)

4.43 ± 1.82 (n = 3416)

4.94 ± 1.48 (n = 636)

13.45 ± 4.13

8.70 ± 5.87 (n = 346)

17.60 ± 8.73 (n = 413)

1.94 ± 1.86

4.63 ± 0.21 (n = 2584)

DIV21

1

2

3

4

5

6

7

18

23

25

32

32

33

39

0 (n = 2968)

5.36 ± 3.40 (n = 598)

3.00 ± 1.33

6.35 ± 3.13 (n = 457)

6.13 ± 3.86 (n = 466)

n.a.

0.6 ± 0.39 (n = 2537)

62.29 ± 3.23 (n = 2455)

68.38 ± 11.07 (n = 573)

56.72 ± 6.65

59.95 ± 11.36 (n = 381)

75.10 ± 13.99 (n = 395)

n.a.

79.89 ± 16.80 (n = 2571)

92.60 ± 7.59 (n = 2968)

90.80 ± 14.67 (n = 598)

70.04 ± 5.16

68.11 ± 17.83 (n = 457)

84.65 ± 14.04 (n = 466)

n.a.

68.90 ± 10.77 (n = 2537)

5.85 ± 0.25 (n = 2455)

7.70 ± 3.29 (n = 573)

5.10 ± 0.73

16.60 ± 5.08 (n = 381)

18.20 ± 7.02 (n = 395)

n.a.

10.33 ± 3.19 (n = 2571)