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Figure 3 | BMC Neuroscience

Figure 3

From: GT1b-induced neurotoxicity is mediated by the Akt/GSK-3/tau signaling pathway but not caspase-3 in mesencephalic dopaminergic neurons

Figure 3

Neurotoxicity of GT1b was not mediated Caspase-3 in neuron-enriched mesencephalic cultures. Activity of caspase-3 was measured by DEVD-AFC cleavage (A), and expressed as the amount of Δ AFC per 1 h in cultures treated with 20 μg/ml GT1b in the absence or the presence of caspase-3 inhibitor (DEVD, 10 μM) at the indicated time points. (B) Number of TH-ip neurons and the levels of [3H]dopamine uptake were assessed in cultures treated with 20 μg/ml GT1b for 24 h in the absence or the presence of caspase inhibitors, z-VAD (100 μM) or DEVD (10 μM). The values represent the mean ± SEM of triplicate cultures in four separate samples; *P < 0.05, **P < 0.001, significantly different from control cultures (ANOVA and Student-Newman-Keuls analyses).

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