Expression analysis of pig Reelin during development. A) Western blot analysis with the monoclonal anti-Reelin antibody Clone 142 on protein samples purified from cerebral cortex of embryonic pigs at the indicated developmental stages. Brain extracts were resolved by PAGE using either a 5% (lower) or 4-15%-gradient gel (upper). Three bands are seen in the samples, of about 400, 320 and ~160 kDa in the 5% gels and 320, ~160, 70, and 65 kDa in the gradient gels. Beta-actin was used as loading control. B) Quantitative analyses of Reelin mRNA expression during pig embryonic brain development. The expression levels were normalized to the expression levels of GAPDH, 18S rRNA and β-actin in the same tissue using the geNorm program.