Skip to main content

Table 1 qRT-PCR primer data

From: Reelin expression during embryonic development of the pig brain

Quantitative real-time PCR primers
Gene Primer sequences Annealing temperature (°C) Amplicon lenght (bp) Accession number
  Forward: 5'-TGC TGT CAC CTT CTG TGA GC-3'    
Reln Reverse: 5'-GAT GGT TGT GCT GAC ATT GG-3' 60 217 XM540392
  Forward: 5'-CCA CGA GAC CAC CTT CAA CT-3'    
ACTB Reverse: 5'-GAT CTT GAT CTT CAT GGT GCT G-3' 60 210 BM190097
  Forward: 5'-CTG ACC GGG TTG GTT TTG AT-3'    
18S rRNA Reverse: 5'-AGC GAG CGA CCA AAG GAA CC-3' 59 134 NR002170
  Forward: 5'-TCA TCA TCT CTG CCC CTT CT-3'    
GAPDH Reverse: 5'-TGT GGT CAT GAG TCC TTC CA-3' 58 202 AF017079