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Figure 2 | BMC Neuroscience

Figure 2

From: Levetiracetam attenuates hippocampal expression of synaptic plasticity-related immediate early and late response genes in amygdala-kindled rats

Figure 2

Identification and verification of differential expressed Pcdh-8, TIEG1 and COX-2 transcripts in the ipsilateral hippocampus of amygdala-kindled rats 3 hrs after the last seizure. Quantitative RT-PCR on total RNA from control and amygdala-kindled rats of A. Pcdh-8, B. COX-2, C. TIEG1 and D. GAPDH. The relative expression level of each cDNA was calculated by the ΔΔ-method, normalized to the expression levels of β-actin in the sample, and set relative to the mean normalized expression levels of the control samples. GAPDH was used as a control. Each quantitative RT-PCR experiment has been done twice of which one representative experiment is shown. Triplicate qPCR data is presented as mean ± SEM. Top inserts A-C, autoradiograms showing the DDRT-PCR bands corresponding to Pcdh-8, COX-2 and TIEG1, respectively. Arrow indicates the DDRT-PCR analysed bands. The 5 lanes correspond to control start, stage 3, stage 5, fully kindled and control end, respectively.

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