Table 2 Primer sequences used in the experiment
mRNA | Accession no.a | primer sequence | Start position b | Product sizec |
|---|---|---|---|---|
ChAT (first round) | XM_001061520 | CAGGAAGGTCGGGTGGACAACATC TCCTTGGGTGCTGGTGGCTTG | 1675 2198 | 524 bp |
GAD67 (first round) | NM_017007 | TTTGGATATCATTGGTTTAGCTGGCGAAT TTTTTGCCTCTAAATCAGCTGGAATTATCT | 762 1162 | 401 bp |
VGLUT1 (first round) | NM_053859 | TACTGGAGAAGCGGCAGGAAGG CCAGAAAAAGGAGCCATGTATGAGG | 188 498 | 311 bp |
VGLUT2 (first round) | NM_053427 | CCCGCAAAGCATCCAACCA TGAGAGTAGCCAACAACCAGAAGCA | 1264 1682 | 419 bp |
Nestin (first round) | M34384 | CTCGGGAGTGTCGCTTAGAG ATTAGGCAAGGGGGAAGGGA | 783 1216 | 434 bp |
ChAT (second round) | XM_001061520 | ATGGCCATTGACAACCATCTTCTG CCTTGAACTGCAGAGGTCTCTCAT | 1840 2163 | 324 bp |
GAD67 (second round) | NM_017007 | CTGACATCAACTGCCAATACCAA GGAGAAAATATCCCATCACCATC | 793 929 | 137 bp |
VGLUT1 (second round) | NM_053859 | GTGGTGGACTGCACTTGCTT CATGTATGAGGCCGACAGTCTC | 274 484 | 211 bp |
VGLUT2 (second round) | NM_053427 | CGCAAAGCATCCAACCAT TGTGGGACCGCAGACAAC | 1266 1529 | 264 bp |
Nestin (second round) | M34384 | GGA GCA GGA GAA GCA AGG GGT CCA GAA AGC CAA GAG | 931 1115 | 185 bp |