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Table 1 Primers used for quantitative polymerase chain reaction

From: Kalrn promoter usage and isoform expression respond to chronic cocaine exposure

Gene Name

Oligo Name

Sequence

Tm (°C)

Length (nt)

GAPDH

mrGapdh-top

TTGTCAGCAATGCATCCTGCACCACC

61

119

 

mrGapdh-rev2bot

CTGAGTGGCAGTGATGGCATGGAC

61

 

3'-terminal exons

   Kalirin7-specific

KalEx33-for

GATACCATATCCATTGCCTCCAGGACC

61

127

 

Kal7unique-rev

CCAGGCTGCGCGCTAAACGTAAG

62

 

   Kalirin8-specific

KalEx38-for

CTGACGTGCCTACTGCTGCAGAC

61

130

 

Kal8unique-rev

CTGGTTGAGGTCCTGGGAGCCAC

62

 

   Kalirin9-specific

KalEx51-for

GCCCCTCGCCAAAGCCACAGC

62

125

 

Kal9unique-rev

CCAGTGAGTCCCGTGGTGGGC

62

 

   Kalirin12-specific

KalEx62-for

CAGCAGCCACGTGCCTGCAGC

62

140

 

KalEx62-rev

TCTTGACATTGGGAATGGGCCGCAC

61

 

Promoter-specific

   Kalirin A promoter

KalPromA-for

CAGGGCAGCCATAAATGGTTTTATCTG

58

121

 

KalEx3-rev

CCTCGCTTGTCACGCCCCC

60

 

   Kalirin B promoter

KalPromB-for

GTGGACGCCTTTTTCCGGACAG

59

116

 

KalEx3-rev

CCTCGCTTGTCACGCCCCC

60

 

   Kalirin C promoter

KalPromC-for

CTTGCTTCGGCTTCTGGATCGAG

59

117

 

KalEx3-rev

CCTCGCTTGTCACGCCCCC

60

 

   Kalirin D promoter

KalPromD-for

CTTTGTTTCTTCCTCACTGCAGCGG

59

120

 

KalEx3-rev

CCTCGCTTGTCACGCCCCC

60

 

   ΔKalirin promoter

ΔKalProm-for

ATCCCAGGGTGAGCAGTGAGAAGG

61

144

 

mKal-Ex11-rev

ATTCCCCAGTCTGAGCCAGCTGC

61

 

   Full-length Kalirins

mKal-Ex10-for

GCCTTTCTCAGCAAACACACTGGGG

61

157

 

mKal-Ex11-rev

ATTCCCCAGTCTGAGCCAGCTGC

61

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