Table 2 Primer pairs used for qRT-PCR expression analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
Ascl1 | AACCGGGTCAAGTTGGTCAA | CGTCTCCACCTTGCTCATCTTC |
Ccnd1 | AGAGGGCTGTCGGCGCAGTA | GGCTGTGGTCTCGGTTGGGC |
Cdk2 | TGCTGAAATGGTGACCCGCAG | TGCCGAGCCCACTTGGGGAA |
Cdk4 | GGTGTATGGCGCCGCAGGAA | GCAGGGGATCTTACGCTCGGC |
p21 (Cdkn1a) | TCCAGGAGGCCCGAGAACGG | CTCCGAACGCGCTCCCAGAC |
p27 (Cdkn1b) | GCACTGTGGAGCAGACGCCC | TCTGCCAGCAGTCCTGGGGT |
p57 (Cdkn1c) | CCAATCAGCCAGCCTTCGACCA | CAGGCGCTGCTACGCGCTAT |
Ctnnb1 | AGGCGAACGGCATTCTGGGC | ACGCACCGTCCTTCGTGCTG |
Gli1 | GCTTGGATGAAGGACCTTGTG | GCTGATCCAGCCTAAGGTTCTC |
Hes1 | TGGCGGCTTCCAAGTGGTGC | GATGACCGGGCCGCTGTGAG |
Hes5 | GCCTGGAGCGGACCAGAGGA | CAGCCGGAGGAGGGAGCCTT |
c-Myc | ACCCGCTCAACGACAGCAGC | CCGTGGGGAGGACTCGGAGG |
Neurod1 | CCGCCACACGCCTACA | CAAACTCGGCGGATGG |
Neurog1 | GACCTGCATCTCTGATCTCG | TGTAGCCTGGCACAGTCCTC |
Olig2 | CTCCGACGCCAAGTGAGCCG | AATCCCCTAGGCCCAGCCCG |
Pax6 | AAGCAACAGATGGGCG | GCTTCATCCGAGTCTTC |
Shh | CACCCCCAATTACAACCCCGACA | TCCAGGCCACTGGTTCATCACAGA |
Sox1 | CTGGGCGCCCTCGGATCTCT | GGACACGGTGCCCGTGAGTG |
Sox2 | GAAAGAAAGGAGAGAAGTTTGGAG | ATCTGGCGGAGAATAGTTGGG |
Sox9 | TCGGTGAAGAACGGACAAGC | TGAGATTGCCCAGAGTGCTCG |
Tcf4 | TGGAGGCCATCCAAGCCCGT | TGCTGGTGGCAACCCTGAACG |