Table 2 Primary antibodies and major biochemical reagents used in this study
Antibody or Biochemical Reagents | Source | Product # | Dilution |
|---|---|---|---|
Mouse anti-PCNA antibody | Santa Cruz | BM 0104 | 1:200 |
Mouse anti-DCX antibody | Santa Cruz | Sc-2024 | 1:100 |
PKH26 | Sigma | PKH26PCL | 1:1000000 |
DAPI | Roche | 46190 | 5 μg/ml |
Alexa Fluor® 488 | Invitrogen | A11055 | 1:200 |
FITC rat IgG 2α κIsotype | Biolegend | 400505 | 1:200 |
APC rat IgG 2α κIsotype ctrl | Biolegend | 400511 | 1:200 |
PE rat IgG 2b κIsotype ctrl | Biolegend | 118419 | 1:200 |
Percp rat IgG 2b κIsotype ctrl. | Biolegend | B118419 | 1:200 |
FITC anti-mouse LY-6A/EC Sca-1 | Biolegend | 122505 | 1:100 |
APC anti-mouse CD34 | Biolegend | 119309 | 1:100 |
PE anti-mouse/human CD44 | Biolegend | 103007 | 1:100 |
Percp anti-mouse CD45 | Biolegend | 103129 | 1:100 |