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Figure 5 | BMC Neuroscience

Figure 5

From: Extended passaging increases the efficiency of neural differentiation from induced pluripotent stem cells

Figure 5

The temporal pattern of proneural gene expression is equivalent in late-passage GG3.1 iPSCs and ESCs, but delayed or suppressed in early-passage cells. (A) The relative mRNA expression of 4 early markers of neural commitment, Ngn1, Msi1, HuC/D and Sox1, and 2 late markers, NSE and Syn, were quantified for undifferentiated, EB and Ni days 3 and 7 cultures. LP cultures showed a significant increase and temporal shift in the expression of most markers, in congruence with ESC expression patterns. (B) The percentage of neural lineage cells in undifferentiated and Ni day 7 EP, LP and ESC cultures as indicated by CD24 cell surface expression. Histograms are representative results of 3 separate experiments are shown. Values are mean ± SD for 2-3 independent samples. *P < 0.05.

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