Microglial culture purity and reduced expression of integrin β-1 by TG G93A microglia. (A and B) Immunostaining of representative mixed glial cultures used to purify microglia. The cultures were stained with anti-CD11b (green) and anti-GFAP (red) antibodies to show microglia in green and astrocytes in red; nuclei were stained with Hoechst (blue). (C and D) Immunostaining with anti-F4/80 antibody of purified resting (C) and activated with lipopolysaccharide (D) microglia. (E) Immunostaining of purified microglia with isotype control antibody shows residual non-specific fluorescence with the isotype-matched antibody. Scale bar = 50 μm. (F) Cell surface integrin β-1 expression levels on NTG (WT), TG WT, NTG (G93A) and TG G93A microglia. Data are presented as mean ± SEM; Student's t test, * p = 0.0493 versus NTG (G93A) cells, n = 14 TG G93A and nine NTG (G93A) mice from four litters, five TG WT and five NTG (WT) mice from two litters.