Figure 5
Reduced intracellular calcium release in TG WT and TG G93A microglia following extracellular ATP stimulation. (A) Intracellular calcium concentration ([Ca2+]i, μM) changes in NTG (WT) and TG WT microglia following extracellular stimulation with 10 μM ATP for 1 min. The period of ATP challenge is indicated with the bar. (B) Changes of [Ca2+]i in NTG (G93A) and TG G93A microglia following extracellular stimulation with 10 μM ATP for 1 min. The period of ATP challenge is indicated with the bar. (C) Area-under-the-curve (AUC) values (μM*s) for NTG (WT), TG WT, NTG (G93A) and TG G93A microglia. Data are mean ± SEM; Student's t test, *p = 0.0176 versus NTG (G93A) microglia. (D) Highest [Ca2+]i values (μM) recorded for NTG (WT), TG WT, NTG (G93A) and TG G93A microglia during 1 min challenge with 10 μM ATP. Data are mean ± SEM; Student's t test, *p = 0.0123 versus NTG (WT) and **p = 0.003 versus NTG (G93A) cells, n = three NTG (WT) mice (38 cells) and three TG WT mice (39 cells) from two litters, three NTG (G93A) mice (50 cells) and three TG G93A mice (40 cells) from two litters.