Figure 1

Dys and Dg are expressed in Drosophila larval and adult nervous systems. Expression pattern for Dg (A-B) and Dys (C-E) in Drosophila 3rd instar larval brains. Photoreceptor neurons are marked with the 24B10 antibody (red). The Dys antibody that recognizes all Dys isoforms by targeting the carboxy terminus shows localization in the brain and the eye discs (C) whereas an antibody that recognizes the Dp186 isoform is present only in the brain (D). Dg (B) and Dys (E) staining is absent in Dg and Dys loss-of-function homozygous mutants respectively. White arrows indicate Dys and Dg signal in the optic lobes and yellow arrows show staining in clusters at the lateral sides of the neuropile. The expression pattern for Dg (F-H) and Dys (I) in the adult brain. Arrows show strong Dg and Dys signals detected in the lamina (La) and medulla (Me) and Dg expression is also seen in the retina (Re). Dys staining is diminished from the brain of Dys loss-of-function homozygous mutant (J). Expression pattern for Dg (K) and Dys (L) in Drosophila motorneurons marked with the 22C10 antibody (green).