- Poster presentation
- Open Access
Quantifying the visual information sourced from melanopsin photoreceptors in mouse LGN field responses
© Siadatnejad et al; licensee BioMed Central Ltd. 2011
- Published: 18 July 2011
- Visual Information
- Field Potential
- Lateral Geniculate Nucleus
- Visual Pathway
- Visual Response
Melanopsin photoreceptors make a third type of photoreceptor along with rods and cones in human and mouse. Until recently melanopsin was thought to participate only in subconscious responses to light (such as pupillary reflexes and regulating the circadian rhythm) and not in image-forming visual responses. Recently, it has been shown that melanopsin derived signals are also widespread in image-forming visual pathways of mice . The goal of this study is to quantify their contribution in the visual pathway. We used information theoretic measures [2, 3] on field potentials  to quantify the amount of information that is originated in melanopsin photoreceptors. The continuous field potentials were recorded from lateral geniculate nucleus (LGN) of transgenic mice (Opn1mwR)  using multichannel electrodes.
The role of melanopsin was quantified by estimating the information  found in LGN responses, about the intensity of constant blue (460nm) and red (655nm) light (7 levels of irradiance were used). The intensities of these two lights were carefully matched to provide equal stimulation of the red-sensitive cones of Opn1mwR animals and to control for the influence of rod photoreceptors. Since the long wavelength (red) light was essentially invisible to melanopsin, subtracting the amount of information in 655nm stimuli from the 460nm should reveal the visual information that is sourced from melanopsin photoreceptors in each recording channel. To investigate the local continuous response signals, the power and phase of recorded field signals were examined at various frequency bands and time points. For each spectrotemporal component of the responses, information about the intensity of light was estimated [1, 3] separately for blue and red lights for each recording channel.
This work was funded by a Wellcome Trust grant to RJL and HDP.
- Brown TM, Gias C, Hatori M, Keding SR, Semo M, Coffey PJ, Gigg J, Piggins HD, Panda S, Lucas RJ: Melanopsin contributions to irradiance coding in the thalamo-cortical visual system. PLoS Biol. 2010, 8 (12): e1000558-10.1371/journal.pbio.1000558.PubMed CentralView ArticlePubMedGoogle Scholar
- Montemurro MA, Senatore R, Panzeri S: Tight data-robust bounds to mutual information combining shuffling and model selection techniques. Neural Comp. 2007, 19: 2913-2957. 10.1162/neco.2007.19.11.2913.View ArticleGoogle Scholar
- Montemurro MA, Rasch MJ, Murayama Y, Logothetis NK, Panzeri S: Phase-of-firing coding of natural visual stimuli in primary visual cortex. Curr Biol. 2008, 18 (5): 375-80. 10.1016/j.cub.2008.02.023.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.