Figure 1From: In vivoassessment of the permeability of the blood-brain barrier and blood-retinal barrier to fluorescent indoline derivatives in zebrafishIn vivo assessment of the permeability of the BBB to fluorescent compounds. Zebrafish larvae (casper line) at 7–8 dpf were immersed in egg water containing 1 μM of an ID or fluorescein for 1 h. In vivo fluorescence imaging of the zebrafish brain was performed using fluorescence microscopes. A: Schematic diagram showing the region observed using the fluorescence microscopes. B-H: In vivo fluorescence imaging of zebrafish larvae stained with ID possessing a rhodanine ring with an acetic acid group (ZMB996, ZMC213, and ZMJ018, B, C and D, respectively), with fluorescein (E), and with ID possessing a rhodanine ring with a propanoic acid group (ZMC808, ZMB740, and ZMB034, F, G and H, respectively). The OT was clearly visualized in zebrafish stained with IDs possessing a rhodanine moiety with a propanoic acid group. Scale bar: 100 μm. OT, optic tectum; CBV, cerebral blood vessel.Back to article page