Effect of hypothermic treatments on the Et-1 induced changes in active caspase-3+-cells in the cortex. (A) shows the active caspase-3+-cells of a normothermic rat, while (B) in a hypothermic animal. Graph (C) shows the overall expression of active caspase-3+-cells of the N group (n = 12) and the H20, H60 and H120 group, (n = 7 in each group), in the ischemic hemisphere. There was no staining in the S and the S + H group (n = 7 and 4 respectively, data not shown), and in the contralateral hemisphere of the other groups. Delaying hypothermia up to 60 minutes after the administration of Et-1, reduced the Et-1 induced apoptosis significantly. There was no binding of the second antibody in the absence of the primary antibody (data not shown). *Significantly different compared to the N group. §Significantly different (p < 0.05) from each other.