Figure 4
PACOCF 3 decreases HuPrP106-126-induced [3 H]-AA release and cell death in primary cortical neurons. (A) Primary cortical neurons were labelled with [3 H]-AA for 24 hours, and subsequently pre-treated for 3 hours with 1 μM PACOCF3 before adding peptides or 1 μM PMA + 5 μM A23187. Cells were lysed after 24 hours and levels of [3 H]-AA measured in supernatant and lysates to calculate a% [3 H]-AA release, ***P < 0.001. (B) Primary cortical neurons were incubated with HuPrP106-126 at varying concentrations for 24 hours (grey bars) or 5 days (black bars). Cell viability was measured using the MTT assay. Data expressed as mean ± S.D. of three observations taken from three experiments (n = 9), ***P < 0.001. (C) Primary cortical neurons were pre-treated for 3 hours with 1 μM PACOCF3 and subsequently exposed to 40 μM HuPrP106-126 for a further 5 days. Cell viability was measured using MTT. Data expressed as mean ± S.D. of three observations from three experiments (n = 9), ***P < 0.001.