Table 2 Primers used in qRT-PCR
Gene symbol | Primer sequence(5’-3’) | Product size (bp) |
|---|---|---|
Nestin (Homo Sapiens) | Forward: TGGCAAAGGAGCCTACTCCAAGAA ; Reverse: ATCGGGATTCAGCTGACTTAGCCT | 111 |
Nestin (Rattus Norvegicus) | Forward: AGAGAAGCGCTGGAACAGAG; Reverse: AGGTGTCTGCAACCGAGAGT | 234 |
Tuj1(Rattus Norvegicus) | Forward: AGCAGATGCTGGCCATTCAGAGTA; Reverse: TAAACTGCTCGGAGATGCGCTTGA | 174 |
Sox2 (Rattus Norvegicus) | Forward: AAAGGAGAGAAGTTTGGAGCCCGA; Reverse: GGGCGAAGTGCAATTGGGATGAAA | 113 |
Vimentin (Rattus Norvegicus) | Forward: AGGTGGATCAGCTCACCAATGACA ; Reverse: TCAAGGTCAAGACGTGCCAGAGAA | 184 |
Map2 (Rattus Norvegicus) | Forward: GCAGCGCCAATGGATTTCCATACA ; Reverse: TCCGTTGATCCCGTTCTCTTTGGT | 104 |
Gapdh(Homo Sapiens) | Forward: CATGTTCGTCATGGGTGTGAACCA; Reverse: AGTGATGGCATGGACTGTGGTCAT | 160 |
Gapdh(Rattus Norvegicus) | Forward: AAGGGCTCATGACCACAGTC; Reverse: GTGAGCTTCCCATTCAGCTC | 169 |