Figure 6

Effects of 1 nM Aβ on α7*-nAChRs in acutely dissociated hippocampal interneurons prepared from GAD67-GFP knock-in mouse. A: Acutely dissociated hippocampal GABAergic interneurons from GAD67-GFP knock-in mice. Phase contrast microscopic image of ventral hippocampus section from a GAD 67-GFP knock-in mouse (Aa). Red labeled region of ventral hippocampus CA1 indicates the area punched out for acute dissociation. GAD 67-GFP expressing interneurons of ventral hippocampus section from a GAD 67-GFP knock-in mouse display green florescence (Ab). Representative phase contrast (Ac) and green florescence images of interneuron (Ad) acutely dissociated from the GAD 67-GFP knock-in mouse ventral hippocampal CA1 area. Scale bar = 250 μm in Aa-b and 10 μm in Ac-d. B. Effects of 1 nM Aβ on choline-induced whole-cell currents in acutely dissociated hippocampal interneurons from GAD67-GFP knock-in mouse. Typical traces illustrate the effects of 1 nM scrambled Aβ (blue traces) or oligomeric Aβ (red traces) on 10 mM choline-induced currents recorded from hippocampal interneurons. The membrane potential was held at −60 mV for these recordings. C: Summary of results of experiments as shown in B. Each symbol was averaged from 5 or 6 cells tested. The vertical bars indicate Mean ± SE. *p < 0.05, **p < 0.01, t-test.