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Figure 4 | BMC Neuroscience

Figure 4

From: Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2Creceptors

Figure 4

Effect of specific antagonists on Ca i ++ release. Cells were plated at 16,000 cells/well for 24 hrs, and loaded with Calcium 4 dye reagent. A. 5-HT2AR- or 5-HT2CR-CHO cells were preincubated with M100907 or SB242084, respectively, for 15 min then stimulated with 1 μM 5-HT. B. 5-HT2CR-CHO cells were preincubated with vehicle or 1 μM SB216641 for 15 min then stimulated with varying concentrations of 5-HT. Inset. Parental CHO-K1 cells (no preincubation) were treated with varying concentrations of 5-HT. n = 3-4 per group.

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BMC Neuroscience

ISSN: 1471-2202

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