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Figure 5 | BMC Neuroscience

Figure 5

From: Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2Creceptors

Figure 5

Time course of ERK activation in 5-HT 2A R- and 5-HT 2C R-CHO cells. Cells were plated at 16,000 cells/well for 24 hrs, preincubated in serum-free medium overnight and stimulated with vehicle (PBS) or 1 μM 5-HT for the indicated times. Levels of pERK were determined by plate immunoassay and net values were determined by subtraction of the vehicle control value from the 1 μM 5-HT value at each time point. Graph is representative of three independent experiments, n = 5-6/experiment; * p < 0.05, ** p < 0.01 vs. t = 0 min.

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